- Datum: –09.30
- Plats: Biomedicinskt centrum C4:2 Seminar room
- Föreläsare: Nicola Crosetto, Science for Life Laboratory, Karolinska Institutet, Stockholm
- Arrangör: Farmbio
- Kontaktperson: Anna Nilsson
Charting the radial organization of the human genome
Chromatin is non-randomly positioned in the cell nucleus with respect to the nuclear interior/ periphery, however, a detailed map of the radial arrangement of eukaryotic genomes remains to be charted. In my talk, I will first describe Genomic loci Positioning by Sequencing (GPSeq), a method that we have developed to systematically infer the distance of DNA loci to the nuclear periphery genomewide. I will demonstrate the use of GPSeq to generate reproducible maps of human genome radiality at one megabase resolution, which revealed that genetic and epigenetic features, gene expression, as well as A/B compartments and chromatin loops are arranged along positional gradients in the cell nucleus. In the second part of my talk, I will present iFISH, a large repository of DNA FISH probes and tools for versatile DNA FISH probe design, which we have built and are continuously expanding, that can be used to investigate multiple aspects of chromosome architecture at the single-cell level.